Table 1.
Comparison of performance of HRP-based LFIAs for the detection of IgG (for reference, conventional AuNP-based LFIAs and ELISAs for IgG are also reported as the first and last table entries, respectively).
Technique | Detection Principle | Tracer | Limit of Detection | Reference |
---|---|---|---|---|
Colorimetric LFIA | Visual detection | AuNP | 50 ng mL−1 | [72] |
Colorimetric LFIA | Measurement of color intensity with a portable strip reader | AuNP | 2 ng mL−1 | [72] |
Colorimetric LFIA | Visual detection | HRP with metal enhancer (AuNPs) using TMB substrate | 5 ng mL−1 | [72] |
Colorimetric LFIA | Measurement of color intensity with a portable strip reader | HRP with metal enhancer (AuNPs) using TMB substrate | 200 pg mL−1 | [72] |
Colorimetric LFIA | Visual detection | HRP with metal enhancer (AuNPs) using AEC substrate | 5 ng mL−1 | [72] |
Colorimetric LFIA | Measurement of color intensity with a portable strip reader | HRP with metal enhancer (AuNPs) using AEC substrate | 310 pg mL−1 | [72] |
Colorimetric LFIA | Visual detection | HRP with metal enhancer (AuNPs) using DAB substrate | 50 ng mL−1 | [72] |
Colorimetric LFIA | Measurement of color intensity with a portable strip reader | HRP with metal enhancer (AuNPs) using DAB substrate | 1.6 ng mL−1 | [72] |
Colorimetric ELISA | Measurements of absorbance using a microwell plate reader | HRP using TMB substrate | 0.24 ng mL−1 | [78] |
Colorimetric ELISA | Measurements of absorbance using a microwell plate reader | HRP using TMB substrate | 20 pg mL−1 | [79] |