Figure 4.

Azelnidipine enhances macrophage mediated phagocytosis of the tumor cells. The effects of the azelnidipine on the phagocytosis of the macrophages were tested by a co-culture of the tumor cells and BMDM cell derived from C57BL/6 or BALB/c mice. GFP⁺ MC38 cells (A), CFSE-labeled CT26 cells (B), and GFP⁺ B16-OVA (C) were co-cultured with the BMDM cells at the ratio of 4:1 in serum-free medium at 37℃ for 4 h with or without the presence of 20 μM of azelnidipine or the positive control anti-CD47 antibody. GFP⁺ or CFSE⁺ BMDMs were detected by a flow cytometry. Representative flow cytometry plots and the summary data of at least three independent experiments were shown. *, p < 0.05, **, p < 0.01, ***, p < 0.001, Student’s t-test.