Figure 1.

α-Humulene attenuates the mucosal lesions in HCl/ethanol-induced gastritis model. Either vehicle (Veh, Saline), α-humulene (α-Hm, 20, 50, or 100 mg/kg) or 50 mg/kg ranitidine was orally administered before intragastric HCl/ethanol administration in rats. (a) Representative images of acute gastric damage and histological investigation by hematoxylin-eosin (H-E) staining of stomach sections. Scale bars are 0.5 mm. (b) Graphs showing the lesion index (arbitrary unit). (c) Representative pictures of Alcian Blue staining of mucus glands. (d) Quantification of Alcian Blue by measuring absorbance at 650 nm after solubilizing the stained tissues. + p < 0.05, ++ p < 0.01, +++ p < 0.001, *** p < 0.001.