Figure 4.

α-Humulene inhibits histamine release in HMC-1 cells. (a) Effect of α-humulene (α-Hm) on histamine release in compound 48/80-treated HMC-1 cells. HMC-1 cells were pre-incubated with α-humulene for 2 h and then stimulated with 5 μg/ml compound 48/80. Histamine levels in the conditioned medium were measured by spectrofluorimetry. Curcumin (50 μM) was used as a positive control. (b) Graphs showing cell viability using the MTS assay. (c) Effect of α-humulene on intracellular calcium influx in HMC-1 cells. (d) Effect of α-humulene on intracellular cyclic adenosine monophosphate (cAMP) levels in HMC-1 cells. + p < 0.05, ++ p < 0.01, +++ p < 0.001, * p < 0.05, ** p < 0.01.