Table 1.
Contribution of PLC-G and NOS-G into rhythmic activity evoked by various hormones in cultured adipocytes (9DIV) of eWAT.
| Agonists and Their Concentrations | |||||||
|---|---|---|---|---|---|---|---|
| ACh 1–5 µM |
NE 1–5 µM |
ANP 1–10 µM |
CCK 3–20 nM |
ANGII 0.2– 1 µM |
BK 0.3–10 µM |
Ins 3–20 nM |
|
| Receptors and proteins |
m3
Gβγ |
α1
Gαq |
NPR-A
Gα |
CCK-B Gαq, Gβγ |
AT-1 Gαq, Gβγ |
B2R Gαq, Gβγ |
RTK TK, cSrc |
|
PLC-G,
% of cells with rhythmic activity |
– | 30–40 | – | 25–40 | 25–40 | 30–40 | 20–30 |
|
NOS-G,
% of cells with rhythmic activity |
70–80 | – | 30–40 | 20–40 | 30–35 | 25–30 | 15–25 |
| Periods of Ca2+-oscillations (s) | 5–60 100–300 |
20–75 100–300 |
20–50 200–300 |
25–30 300–500 |
20–50 75–200 |
10–30 200–500 |
20–30 50–150 |