Figure 2.
Influence of quercetin on mRNA expression for angiogenic factors in mast cells in vitro. Mast cells sensitized with IgE (1 × 105 cells/mL) were stimulated with 10.0 ng/mL specific antigens in the presence of various concentrations of quercetin for 12 h. The levels of mRNA expression for VEGF (A), bFGF (B), TNF (C), IL-6 (D) and IL-8 (E) in mast cells were examined by real-time RT-PCR. One representative experiment of two is shown. VEGF: vascular endothelial growth factor; bFGF: basic fibroblast growth factor; TNF: tumor necrosis factor-α; OVA: ovalbumin; and N.S.: not significant (p > 0.05) vs. OVA. * Significant (p < 0.05) vs. med. alone. ** Significant (p < 0.05) vs. OVA and quercetin (2.5 µM).