Table 11.
List of primers used in real-time PCR.
| Gene | Forward | Reverse |
|---|---|---|
| 18s | CGGCTACCACATCCAAGGA | AGCTGGAATTACCGCGGC |
| Gp | TGGCAGAAGTGGTGAACAATGAC | CCGTGGAGATCTGCTCCGATA |
| Adiponectin | AGAGTCGTTGACGTTATCTGCATA | GGGCTCTGTGCTGCTCCATCT |
| Gs | ACTGCTTGGGCGTTATCTCTGTG | ATGCCCGCTCCATGCGTA |
| Atgl | GAGCCCCGGGGTGGAACAAGAT | AAAAGGTGGTGGGCAGGAGTAAGG |
| Hadh | ACTACATCAAAATGGGCTCTCAG | AGCAGAAATGGAATGCGGACC |
| Cd36 | TGGCCTTACTTGGGATTGG | CCAGTGTATATGTAGGCTCATCCA |
| Hbdh | AGTTTGGGGTCGAGGCTTTC | TGGTGGCCGCTATGAAGTTG |
| F480 | CTTTGGCTATGGGCTTCCAGTC | GCAAGGAGGACAGAGTTTATCGTG |
| Mct1 | GCCTGAGCAAGTCAAGCTAG | TCAGACCTCGGATCCAGTAC |
| Il-6 | AACGATGATGCACTTGCAGA | TGGTACTCCAGAAGACCAGAGG |
| Oxct1 | CCAAGGAAGTAAATGAAGATCTCCTA | ACGTGTATGTTACAAGAAATGGCTTACC |
| Klotho | TGTTCTGCTGCGAGCTGTTAC | TACCGGACTCACGTACTGTTT |
| Tfam | TTCCCAAGACTTCATTTCATTGTC | GATGATTCGGCTCAGGGAAA |
| Leptin | GCTTTGGTCCTATCTGTCTTATGTT | CAATGGTCTTGATGAGGGTTTT |
| Cpt1α | CCAGGCTACAGTGGGACATT | GAACTTGCCCATGTCCTTGT |
| Prdm16 | CCACCAGCGAGGACTTCA | GGAGGACTCTCGTAGCTCGAA |
| Mcad | GCTCGTGAGCACATTGAAAA | CATTGTCCAAAAGCCAAACC |
| Pgc1α | GACTGGAGGAAGACTAAACGGCCA | GCCAGTCACAGGAGGCATCTTT |
| Cxcl2 | CCAACCACCAGGCTACAGG | GCGTCACACTCAAGCTCTG |
| Pparγ | CCACCAGCGAGGACTTCAC | GGAGGACTCTCGTAGCTCGAA |
| Ucp1 | TGGTTGGTTTTATTCGTGGT | AGGGTTTGTGGCTTCTTTTC |
| Cidea | TCCTCGGCTGTCTCAATG | GGCTGCTCTTCTGTATCG |
| Fgf21 | CAAGACACTGAAGCCCACCT | CACCCAGGATTTGAATGACC |
| Pparα | GAACCGGAACAAATGCCAGT | CTTCAGGTAGGCTTCGTGGA |
| Il-10 | GCTCTTACTGACTGGCATGAG | CGCAGCTCTAGGAGCATGTG |
| Sirt1 | GCAACAGCATCTTGCCTGAT | GTGCTACTGGTCTCACTT |
| Cs | GCAGCCAAGAACTCATCCTG | TCTGGGCCTGCTCCTTAGGTA |
| Acat1 | CCGAGACAACTACCCAAGGA | CACACACAGGACCAGGACAC |
| Cox4 | TGGGAGTGTTGTGAAGAGTGA | GCAGTGAAGCCGATGAAGAAC |
| Cytochrome c | CACGCTTTACCCTTCGTTCT | CTCATTTCCCTGCCATTCTC |
| Hk2 | CTGTCTACAAGAAACATCCCCATTT | CACCGCCGTCACCATAGC |
| Pepck | ACAGTCATCATCACCCAAGAGC | CATAGGGCGAGTCTGTCAGTTC |
| Acc2 | GACGCCCGAGGATCTGAAG | GGGACAGGGACGTACTGATC |
| Hmgcs 2 | AACTGGTGCAGAAATCTCTAGC | GGTTGAATAGCTCAGAACTAGCC |
18s, 18s ribosomal RNA; Gp, glycogen phosphatase; Gs, glycogen synthase; Atgl, adipose triglyceride lipase; Hadh, hydroxyacyl-coA dehydrogenase; Cd36, cluster of differentiation 36; Hbdh, 3-hydroxybutyrate dehydrogenase; Mct1, monocarboxylate transporter 1; Il-6, interleukin-6; Oxct1, 3-oxoacid CoA-transferase 1; Tfam, mitochondrial transcription factor A; Cpt1α, carnitine palmitoyltransferase 1 alpha; Prdm16, PR domain containing 16; Mcad, medium chain acyl-CoA dehydrogenase; Pgc1α, peroxisome proliferator activated receptor gamma coactivator-1 alpha; Cxcl2, chemokine (C-X-C motif) ligand 2; Pparγ, peroxisome proliferator activated receptor gamma; Ucp1, mitochondrial uncoupling protein 1; Cidea, cell death inducing DNA fragmentation factor-alpha like effector A; Fgf21, fibroblast growth factor 21; Pparα, peroxisome proliferator activated receptor alpha; Il-10, interleukin-10; Sirt1, sirtuin1; Cs, citrate synthase; Acat1, acetyl-coenzyme A acetyltransferase 1; Cox4, cytochrome c oxidase polypeptide IV; Hk2, hexokinase 2; Pepck, phosphoenol-pyruvate carboxykinase; Acc2, Acetyl-CoA carboxylase 2; Hmgcs2, hydroxy-methylglutaryl-CoA synthase 2.