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. 2021 May 10;11(5):712. doi: 10.3390/biom11050712

Figure 3.

Figure 3

BDNF-mediated MC viability. (A) Viability of rMC1 cells cultured in NG or HG media with or without rBDNF. A: rBDNF-mediated rMC1 cell viability in a dose-dependent manner under a diabetes-like condition. (B,C) Quantitative RT-PCR and immunoblotting analysis of rMC1 cells 48 h after TrkB siRNA and scramble siRNA control (Ctrl) transfection. (D,E) Analysis and representative images of immunoblotting for pAKT and tAKT (D) and pERK and tERK (E) in rMC1 cells transfected with TrkB siRNA and scramble control siRNA 48 h post-transfection in HG media. (F) rBDNF (25 ng/mL) rescued HG-induced loss of rMC1 cells transfected with TrkB siRNA and scramble control siRNA. Graph bars represent mean ± SEM. *: p < 0.05; **: p < 0.01; ***: p < 0.001. TrkB knockdown significantly reduced its mRNA and protein levels and MC viability under diabetic conditions.