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Antibody dilution factors in immunoblotting (IB) and immunohistochemistry ((HC).
| Target | Primary Antibody Dilution | Secondary Antibody Dilution |
|---|---|---|
| β-Actin (IB) | 1:5000 | 1:4000 |
| AKT (IB) | 1:1500 | 1:4000 |
| pAKT (IB) | 1:2000 | 1:4000 |
| BDNF (IB) | 1:1000 | 1:4000 |
| proBDNF (IB) | 1:1000 | 1:4000 |
| ERK (IB) | 1:1500 | 1:4000 |
| pERK (IB) | 1:2000 | 1:4000 |
| TRK-B (IB) | 1:1000 | 1:4000 |
| BrdU (IHC) | 1:1000 | 1:700 |
| GS (IHC) | 1:1000 | 1:700 |
