Figure 4.

(A,B) Representative confocal images showing OB* (A) and SF (B) incubated in a glass coverslip at 0.3 μM in SH-SY5Y culture medium without cells for 0 and 24 h at 37 °C. The green-fluorescent signals arise from the staining with the A11 and 211 antibodies, respectively. The pink and yellow boxed areas show higher magnifications of the αS species. (C) Semi-quantitative analysis of the oligomeric αS-derived green fluorescent signal showed in panels A and B, and expressed as number of puncta per µm². Error bars indicate S.E.M. The statistical analysis was made by Student t-test relative to samples of SF at time 0 h (°°° p < 0.001). (D) Western Blotting of the cytosolic (cyto) and membrane (mem) fractions purified from SH-SY5Y cells treated for 24 h with SF or OB* at 0.3 μM. αS species were then probed with conformation-insensitive anti-αS 211 antibodies (Original Western Blot Figure see Figure S1).