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. 2021 May 9;9(5):526. doi: 10.3390/biomedicines9050526

Figure 4.

Figure 4

Schematic presentation of mechanism of hTERT pre-mRNA splicing induced by EndoG. (A) Hypothetical locations for synthesis of hTERT pre-mRNA and lnc-RNA. Pre-mRNA hTERT (blue dotted line) is synthesized from coding strand of hTERT gene, while lnc-RNA (red dotted line) is synthesized from the noncoding DNA strand. (B) Hypothetical schematic locations for lncRNAs and EndoG-produced oligonucleotides (EGPOs) during regulation of hTERT pre-mRNA AS by EndoG. EndoG (white box) cleaves EGPO (red bold font) from lnc-RNA (red font), which is complementary to hTERT pre-mRNA. (C) Binding sites (blue bold font in gray boxes) for the SRp20 (green box) and SRp40 (purple box) splicing regulatory proteins are located in intron 8. (D) Interaction between EGPO and hTERT pre-mRNA prevents binding of SRp20 and SRp40 to hTERT pre-mRNA, which results in induction of AS and expression of truncated β- splice variant.