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. 2021 May 7;13(5):851. doi: 10.3390/v13050851

Table 1.

PCR primer and probe sequences.

Primer/Probe 1 Sequence (5′ → 3′)
Nikisins F2 MORV AAT CAA TTT GTG AAT GTG CCA
Nikisins R MORV GCT CAG GTT TCA TAT AGT TTA GAC CA
Nikisins TM MORV /56-FAM/AAG TGG GGC/ZEN/CCA ATG ATG TCC CCA TT/3′ IB®FQ/
Nikisins F2 MOBV AAC CAA CTT ATG GAT ATG CCA
Nikisins R MOBV TGG GCC TTC TAT CTT ATA GCC TGG ACC A
Nikisins TM MOBV /56-FAM/AAT GGG GGC/ZEN/CTA TGA TGA CCC CCT T/3′ IB®FQ/

1 Probes = 250 nm PrimeTime® 5′ 6-FAM™/ZEN™/3′ IB®FQ.