Table 1.
Primer/Probe 1 | Sequence (5′ → 3′) |
---|---|
Nikisins F2 MORV | AAT CAA TTT GTG AAT GTG CCA |
Nikisins R MORV | GCT CAG GTT TCA TAT AGT TTA GAC CA |
Nikisins TM MORV | /56-FAM/AAG TGG GGC/ZEN/CCA ATG ATG TCC CCA TT/3′ IB®FQ/ |
Nikisins F2 MOBV | AAC CAA CTT ATG GAT ATG CCA |
Nikisins R MOBV | TGG GCC TTC TAT CTT ATA GCC TGG ACC A |
Nikisins TM MOBV | /56-FAM/AAT GGG GGC/ZEN/CTA TGA TGA CCC CCT T/3′ IB®FQ/ |
1 Probes = 250 nm PrimeTime® 5′ 6-FAM™/ZEN™/3′ IB®FQ.