Figure 5.
(a) FTIR absorbance spectra of E. coli antibodies immobilized on MHDA/MUDO (1:9) SAM-coated GaAs samples following 1-h exposure to 100 μg/mL antibody suspensions at two different pH conditions. Each spectrum represents an example of one of 3 tested samples; fluorescence images of GFP E. coli bacteria immunocaptured after 1-h exposure to E. coli suspensions at 106 CFU/mL. The E. coli antibodies were immobilized following 1-h incubation in suspensions at 100 μg/mL in two different buffers: (b) acetate (pH 4.5) and (c) PBS (pH 7.4).