Table 4.
Culturing approaches to favor specific microbiota species and NGP taxa and candidatus species.
| Reference/Sample | Culture Media | Culture Media Modifications | Selected Favored Cultured Microorganisms | Outcome and Observations: New Species Cultured: Potential NGP |
|---|---|---|---|---|
| Browne et al. [118] Human |
YCFA | Glucose (0.2%), maltose (0.2%), and cellobiose (0.2%) | Aero-intolerant genus and species | 68 new isolated species: 16S RNA similarity 86–97% Anaerotruncus colihominis Blautia luti; B. hydrogenotrophica Clostridium boltae; C. celerecrescens; C. celerescens; C. clostridioforme; C. cocleatum; C. disporicum; C. ghonii; C. hathewayi; C. innocuum; C. lituseburense; C. methylpentosum; C. nexile; C. oroticum; C. saccharogumia; C. saccharolyticum; C. thermocellum; C. xylanolyticum Coprococcus eutactus Oscillibacter valericigenes Roseburia faecis; R. inulinivorans Ruminococcus albus; R.bromii; R. flavefaciens; R. gnavus; R.obeum; R. torques |
| YCFA | Pre-treatment with ethanol 70% (v/v), glucose (0.2%), maltose (0.2%), cellobiose (0.2%), sodium taurocholate (0.1%). Spore-forming gut aero-intolerant bacteria |
Alistipes finegoldii
Anaerotruncus colihominis Blautia hydrogenotrophica; B. obeum; B. wexlerae Clostridum baratti; C. bartlettii; C. clostridioforme; C. disporicum; C. hathewayi; C.innocuum; C. paraputrificum; C.perfringens Coprococcus comes; C. eutactus Prevotella copri Roseburia hominis; R. intestinalis; R. inulinvorans; Ruminococcus bromii; R. gnavus; R. obeum; R. torques |
||
| Chang et al. [119] Human |
YCFA | Pre-incubation in blood culture bottles supplemented with 10% sheep blood and 10% rumen | Aero-intolerant bacteria Alistipes shahii; A. onderdonkii, Clostridium bifermentans, C. innocuum, C. hiranonis, C. butiricum, C. hathewayi, C. bolteae, C. sporogenes, Odoribacter splanchnicus |
22% of species isolated increase: 16S RNA similarity 93–97% 3 new species isolated: Longicatena caemuris Bacillus alcalophilus Pseudogracilibacillus auburnensis |
| Gotoh et al. [120] Microbial bank |
GAM | NA | Aero-intolerant bacteria 72% of species of the top 56 species listed in the “human gut microbial gene catalogue” cultured in GAM |
Isolated species in GAM: Anaerotruncus colihominis, Blautia hansenii, Clostridium nexile, C. asparagiforme, C. scindens, Coprococcus comes Roseburia intestinalis Ruminococcus torques, R. lactaris, R. obeum, R. gnavus. |
| Lagier et al. [121] 16-years-old male |
BHI | Preincubation of the stool with lytic E. coli T1 and T4 phages | Non-fastidious aerobic and facultatively anaerobic bacteria | Enterobactermassiliensis strain JC163T |
| Bailey and Coe [122] Rhesus Monkeys |
BHI | NA | Non-fastidious aerobic and facultatively anaerobic bacteria | NA |
| EMB | NA | Gram-negative aerobic and facultatively anaerobic bacteria | NA | |
| LBS | NA | Aerobic members of lactobacilli | Lactobacillusspp. | |
| Lei et al. [123] Female mice |
GMM | NA | Gut aero-intolerant bacteria | |
| López-Moreno [117] | BHI | Supplemented with Obesogens: BPA, BPS | Anaerobic facultative Firmicutes | Staphylococcus, Bacillusamyloliquefaciens group, Streptococcussalivarius |
| López-Moreno [117] | MRS | Supplemented with Obesogens: BPA, BPS | Lactobacillus, Enterobacteria | Latilactobacillus sakei, Enterococcus faecium |
YCFA: yeast-extract-casein hydrolysate-fatty acids; GAM: gifu anaerobic medium; BHI: brain–heart infusion; EMB: eosin methylene blue; LBS: Lactobacillus selection; GMM: gut microbiota medium; MRS; Man, Rogosa and Sharpe; BPA: Bisphenol A; BPS: Bisphenol S. Genera and species in bold letters highlight the microorganisms to be considered as potential NGP to be searched, cultured and assayed for their anti-obesity modulation effects.