Table 3.

Effect of EtOH on radiolysis during high activity 68Ga labeling of lyophilized NOTA-sdAb.

Compound	EtOH (%)	Activity (GBq)	Method	QC/iTLC (%)						pH
				10 min			3 h
NOTA-anti-MMR Lyo				1	2	3	1	2	3
	0	1.06	iTLC	69	17	14	65	26	9	4.82
	20	1.17	iTLC	80	13	7	82	16	2	4.59

The iTLC strips were scanned and three main peaks were observed. 1 = Rf = 0 [⁶⁸Ga]Ga-NOTA-sdAb; 2 = Rf = 0.7 radiolysis compound; 3 = Rf = 1 unlabeled ⁶⁸Ga (⁶⁸Ga-citrate); RCP = Radiochemical Purity; iTLC = instant Thin Layer Chromatography; Mass precursor used per labeling = 100 µg; % EtOH = % ethanol in the labeling buffer, before addition of ⁶⁸Ga eluate.