Table 7.
Osmolality of different conditions.
| Sample | Volume (mL) | NOTA-sdAb (mg/mL) | Buffer Final Concentration | Kit Excipients * | EtOH (%) | AA (mg/mL) | Osmolality (mOsm/kg) ½ Diluted | Osmolality (mOsm/kg) |
|---|---|---|---|---|---|---|---|---|
| Reference | 2.2 | 0.09 | 0.5 M NaOAc pH 5 | / | / | / | 405, σ = 3.8 | 811, σ = 7.6 |
| Solution 1 | 2.2 | 0.09 | 0.5 M NaOAc pH 5 | Yes | / | / | 460, σ = 3.5 | 921, σ = 7.0 |
| Solution 2 | 2.2 | 0.09 | 0.5 M NaOAc pH 5 | Yes | 10% | / | 1248, σ = 2.9 | 2495, σ = 5.8 |
| Solution 3 | 2.2 | 0.09 | 0.5 M NaOAc pH 5 | Yes | 10% | 2.27 | 1349, σ = 13.5 | 2698, σ = 27.1 |
| 10 | 0.02 | 0.5 M NaOAc pH 5 | Yes | 10% | 0.5 | 1343, σ = 10.1 | 2685, σ = 20.2 | |
| Solution 3 (1:3 dilution with WFI) | 30 | 0.007 | 0.017 M NaoAc pH 5 | Yes | 3.33% | 0.17 | / | 1088, σ = 5.44 |
* kit excipients: sucrose, mannitol and polysorbate 80; AA = ascorbic acid.