Figure 1.

Schematic overview of transposase-mediated cut-and-paste transposition. A gene of interest (GOI, black bar) is mobilized by transposase (Tnpase) molecules (grey amorphous shape) from vector donor DNA (grey DNA stands) to a genomic locus (blue-red DNA strands). The transposase binds to the terminal inverted repeats (TIRs, grey arrows), induces double-stranded breaks (indicated with yellow lightning bolts), and excises the mobile element from the donor DNA leaving behind a footprint. The transposon-transposase complex finds a suitable target site (TS) and performs integration, producing a target site duplication (TSD).