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. 2021 May 12;10(5):1183. doi: 10.3390/cells10051183

Figure 2.

Figure 2

Level of CD40L expression and number of CD40L feeder cells regulates differentiation of human naive B-cell into CD27+CD38− B-cells and CD27+CD38+ antibody-secreting cells. (A) Representative of FACS plot showing CD27 and CD38 expression among human naive B-cells cultured on CD40L-expressing 3T3 cells (as in Figure 1A) with or without IL-21 and/or IL-4 for 11 days. (B,C) The frequency of CD27+CD38− (B) and CD27+CD38+ (C) populations were analyzed 11 days after culture on CD40L-expressing 3T3 cells and cytokines. (D) Representative plots of human naive B-cells cultured on 0/1000/10,000 CD40L+++-expressing 3T3 cells (as in Figure 1A) supplemented to 10,000 with WT with or without IL-21 and/or IL-4 for 11 days. (E,F) The frequency of CD27+CD38− (E) and CD27+CD38+ (F) cells 11 days after culture on different numbers of CD40L+++-expressing 3T3 cells and cytokines. Data are shown as the mean ± SEM. (n = 6 (A–C) and n = 7 (D–F) independent experiments). Single experiments were conducted in triplicate. Data were analyzed by a two-way ANOVA followed by Tukey’s multiple comparison test. * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001, **** P ≤ 0.0001.