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Adhesion molecule gene transcription in A. vasorum antigen (AvAg)-stimulated canine endothelial cells. CAEC were stimulated with soluble A. vasorum L3 antigen (AvAg; 1 ng/mL) and after 3, 6, 12 and 24 h of incubation total RNA was isolated, reverse transcribed and assayed for E-selectin (A), P-selectin (B), ICAM-1 (C) and VCAM-1 (D) gene transcription via qPCR. Data are expressed as n-fold of controls (non-stimulated CAEC). TNF-α stimulation was used as positive control.
