Table 2.
Primers used in this study.
| Primer Name | Forward Primer (5′–3′) | Reverse Primer (5′–3′) |
|---|---|---|
| cDNA cloning | ||
| PtCLec2 | CCGAGAATGCGAATCTACTTGC | TGTTTACTGTGAGCAGCAGCCAC |
| RACE | ||
| PtCLec2-3P1 | CGACCAACGCATCGCCACCAG | |
| PtCLec2-3P2 | CGACATTGCCTGCTACCACAAGAAG | |
| PtCLec2-5P1 | AGCCTGGTGGCGATGCGTTGG | |
| PtCLec2-5P2 | TCAGCCAGTCAACCTTGCTACCTTCC | |
| UPM (long) | CTAATACGACTCACTATAGGGCAAGCAGTGGTATCAACGCAGAGT | |
| UPM (short) NUP |
CTAATACGACTCACTATAGGGC AAGCAGTGGTATCAACGCAGAGT |
|
| Recombinant expression | ||
| RE-PtCLec2 | CGCGGATCCCAAGGCCGTGTGTTGGCG | CCGCTCGAGACCAAAATGTGCTCGTGCGCG |
| qRT-PCR | ||
| PtCLec2-qRT | ACAGGACAACTGGAAGGTAGCAAG | GAGAAGAACCATCCCTTGACACTG |
| PtcSP1-qRT | ACTATGTCCAGCCAGCGTGT | GGAAGGACTCGCGCTCATAG |
| PtcSP2-qRT | TAAGGACATCGGACAGGAGACACT | TAAGGAAGTGAACGCTATCTCT |
| PtcSP3-qRT | AAGCCAGTCGAAATACAGGAG | CAGCATCTCCTTCCCAATTCC |
| PtSPH-qRT | CATCCTTGACCAGCCAGCA | CCCACCCAGACACAACACA |
| PtMyosin-qRT | CGTTGGCGAAGTAGGAGAGT | GAACAAGAGGCGTAATGAGGT |
| PtRab5-qRT | AACCCAGCATCCAGTCACCC | TACCCCTAAGCCCCTCAACC |
| PtArp-qRT | GCTCCACCATCACCACTCTG | TTAGCCATCTCCTTCCCTGC |
| PtproPO-qRT | CCTCTTCTTCACGACACTCAACTG | TCACGAGATAACACAAAACGCC |
| PtPPAF-qRT | GGACAGGACCAAGACCCAGT | GATTTGAGAAGGAACAAGCGTG |
| Ptα2M1-qRT | TGTGCCTCCTACCGCCTTCC | GGTGTCCCTCTCTCTCAACTCATTT |
| Ptα2M2-qRT | GTGGTTGGCTACGGGACGGGT | ACGGCAATGTCATCACTGGGGAT |
| PtTEP-qRT | CTCTTCTCGCTGCTTTCCTTCATC | TTTTTGGGACTTTGCCACCACTT |
| PtCrustin1-qRT | GGCAGTTGTGGCTACCATTGT | CGCTCGGTGTAAGGTGGATAG |
| PtCrustin2-qRT | TGGTGATGGCGGTGGCTATG | TCCTTGGCGAACATCCTCT |
| PtCrustin3-qRT | AGTATCTCAGAATCGACCA | CCCTCTTAGTTTCTCTTGTT |
| PtALF1-3-qRT | ACGACGAGGAGGAGAAAGAGG | GGCACTGATGGTGGAAACTGA |
| PtALF4-qRT | GACGCTCTGAAGGACTTTATG | CGCCGAAACGCTTAGAAATAC |
| PtALF5-qRT | TAGTCGTGGTGAGAGGGCAA | CTTTGCTCTCTCATCAGGAC |
| PtALF6-qRT | CATACTCCCGTGAACTCCTA | CAGACCGTGATTTGTGGAG |
| PtALF7-qRT | GCATTTTCTATTTTCCTATCC | GCATGAGTCTTGTATATTTGG |
| PtMBL-qRT | GGCACCGTCGGTCATCCAAC | GCTGGCTGCGACCAAACCTT |
| PtJNK-qRT | AGTGTGGCGGCTCAGCTGTT | CTCCACTCCGACTGCCTCGC |
| PtRelish-qRT | CCAGAGTACGCAAGCCACATCAC | CCGCAGCACCACCTTGTTCAG |
| PtPelle-qRT | ACTCTTGCCTTCCCTTGCTAAC | ACTGACCATGAATCATACCCCTG |
| PtMyD88-qRT | GGTCCTTGAAGCAACAGGTGGTAG | AGTGCTGGCTGACTAGGAGATGAC |
| PtTLR-qRT | CATTGAGGACAGCCACAGGAC | TGGTAGAGAGGTACAGCTTGAGTTC |
| β-actin-qRT | TCACACACTGTCCCCATCTACG | ACCACGCTCGGTCAGGATTTTC |
| RNAi | ||
| PtCLec2-siRNA | GACGCAACAUCACGUACAU | |
| GFP-siRNA | GGAGGACGGCAACAUCCUG | |
| Sequencing | ||
| M13 | CGCCAGGGTTTTCCCAGTCACGAC | GAGCGGATAACAATTTCACACAGG |
| T7 | TAATACGACTCACTATAGGG | GCTAGTTATTGCTCAGCGGT |
The BamH I and Xho I sites are underlined.