Figure 3.

Differential inhibition effects of PLB on cell viability and migration in A375 and SK-MEL-28. (a) A375 and SK-MEL-28 cells were seeded in the 96-well plate at the density of 1 × 10⁴ cells/well and treated with PLB (DMSO and 1, 2, 2.5, 3, 3.5, 4, and 6 µM PLB for A375 cells; DMSO and 1, 2, 4, 6, 7, 8, and 10 µM PLB for SK-MEL-28 cells) for two days. Viable cell numbers were measured by MTS assay. The IC50 of A375 and SK-MEL-28 cells were 2.790 and 3.872 µM, respectively (n = 3). (b) A375 cells and (c) SK-MEL-28 cells treated with DMSO and 1 and 3 µM PLB were counted for 5 days (n = 2). (d) Two cell lines were seeded in a 6-well plate, and the migration status was displayed by different treatments (DMSO and 1 and 3 µM PLB) and time points (0, 6, 12, 24, and 48 h). The migration inhibition rates in the 1 and 3 µM PLB groups were compared with the DMSO group. Scale bar = 0.1 mm. (e) Migration inhibition rate of A375 cells. (f) Migration inhibition rate of SK-MEL-28 cells (n = 3). Results are expressed as mean ± SD. MTS, nonradioactive cell proliferation assay; IC50, 50% inhibitory concentration.