Table 1.
The primers for qPCR.
| Genes | Primer Sequence (5’–3’) | Product Size (bp) | Tm (°C) |
|---|---|---|---|
| Actb | Forward: CCAGCCTTCCTTCTTGGGTAT | 93 | 60 |
| Reverse: AGGTCTTTACGGATGTCAACG | |||
| Lhcgr | Forward: CTGAGGAGATTTGGTTGCTGTA | 234 | 60 |
| Reverse: ATTTGGGTGGACTTTTTTGGGG | |||
| Cyp11α1 | Forward: GGGCAGTTTGGAGTCAGTTTAC | 186 | 60 |
| Reverse: TTTAGGACGATTCGGTCTTTCTT | |||
| Fshr | Forward: GCAGATGTGTTCTCCAACCTACC | 172 | 60 |
| Reverse: GGAGAGACTGGATCTTGTGAAAGG | |||
| Egfr | Forward: AAGGCACAAGTAACAGGCTCA | 114 | 60 |
| Reverse: CCAAGTTCCCAAGGACCACT | |||
| Cyp19α 1 | Forward: GACACATCATGCTGGACACC | 179 | 60 |
| Reverse: CAAGTCCTTGACGGATCGTT | |||
| Vegfα | Forward: GAGAAGACAGGGTGGTGGAAG | 193 | 60 |
| Reverse: GAAGGGAAGATGAGGAAGGGT | |||
| Vegfr2 | Forward: CCTGCCTACCTCACCTGTTTC | 205 | 60 |
| Reverse: CCACTGTCTGTCTGGCTGTC | |||
| Inhα | Forward: CTTTCCCTCTGCTGACCCA | 184 | 60 |
| Reverse: AAAGCCGCAGGAGACCAA | |||
| CyclinD2 | Forward: GCTATGGAGCTGCTGTGCT | 263 | 60 |
| Reverse: CCAAGAAACGGTCCAGGTAA | |||
| PCNA | Forward: ACCTGCAGAGCATGGACTCG | 83 | 60 |
| Reverse: GCAGCGGTATGTGTCGAAGC | |||
| P21 | Forward: CCTGGTGATGTCCGACCTG | 130 | 60 |
| Reverse: CCATGAGCGCATCGCAATC | |||
| P27 | Forward: TCAAACGTGAGAGTGTCTAACG | 238 | 60 |
| Reverse: CCGGGCCGAAGAGATTTCTG |