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. 2021 May 12;13(5):892. doi: 10.3390/v13050892

Figure 4.

Figure 4

PERK impacts early events of VEEV replication. (A,B) Primary astrocytes were transfected with 100 nM of siNeg or siPERK siRNAs prior to VEEV TC-83 infection (MOI of 5). (A) Supernatants were collected at the indicated time points and viral titers were determined via plaque assay. (B) Cell lysates were collected at the indicated time points. RNA extraction was performed, and viral genomic copies were determined by RT-qPCR. Data are expressed as the mean  ±  SD (n  =  3). ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001.