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. 2021 May 12;13(5):892. doi: 10.3390/v13050892

Figure 5.

Figure 5

Loss of PERK does not result in elevation of interferon beta (IFN-β) production. Primary astrocytes were transfected with 100 nM of siNeg or siPERK siRNAs. At 48 h post transfection, (A) Cells were infected with VEEV TC-83 (MOI 5). Total RNA was isolated at 3, 6, 9, 12, and 18 hpi. IFN-β gene expression was determined by RT-qPCR. Fold changes were calculated relative to 18S ribosomal RNA and normalized to mock samples using the ΔΔCt method. Data are expressed as the mean  ±  SD (n  =  3). (B) Cells were treated with poly(I:C) complexed with Lipofectamine 2000 for 6 h. siNeg transfected cells treated with Lipofectamine only was used as a control. IFN-β gene expression determined as described above. Data are expressed as the mean  ±  SD (n  =  3). * p ≤ 0.5, *** p ≤ 0.001.