Figure 2.

Comparison among visualization techniques. The left column shows the process of RGB coloring. The first three panels show the original three excitation wavelengths images chosen for false coloring, the same three images with false-color look-up tables applied, and the resultant composite image generated when the false-colored images are merged. The center column shows the component-wise false-colored results of spectral unmixing performed with the library taken from the Thermo Fisher Scientific Spectra Viewer. The right column shows the same component-wise false-colored results of spectral unmixing when performed with the library generated from the excitation-scanning spectral microscope. False color look-up tables applications for the center and right columns are as follows: blue NucBlue, magenta Fura-2, red Fura Red, cyan Calcein Green, green MitoTracker Green, yellow TRITC WGA, and an overlaid composite of these images.