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. Author manuscript; available in PMC: 2021 May 26.
Published in final edited form as: Proc SPIE Int Soc Opt Eng. 2019 Mar 4;10881:108811B. doi: 10.1117/12.2510784

Figure 3.

Figure 3.

Libraries used for sub-selected spectral unmixing. All values were normalized to a value of unity at the wavelength with the strongest signal. The coloring is identical for all three plots: Autofluorescence (black), NucBlue (blue), TRITC WGA (orange), Fura-2 (purple), Fura Red (red), and MitoTracker Green (green). (Top) The excitation-scanning library with normalized intensity values sub-selected every 20 nm. (Middle) The excitation-scanning library with normalized intensity values sub-selected every 40 nm. (Bottom) The excitation-scanning library with normalized intensity values sub-selected to correspond to the values containing the most intense excitation wavelength per library component.