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. 2021 May 12;26(10):2870. doi: 10.3390/molecules26102870

Figure 3.

Figure 3

The effects of LOS-ME on TNF- α, IL-1β, and IL-6 mRNA expression in LPS-activated BV2 microglial cells. (A) Measurement of the expression level of proinflammatory cytokines (TNF-α, IL-1β, and IL-6) on mRNA level used RT-PCR (B)TNF-α mRNA expression with corresponding fold change and (C) IL-1β mRNA expression with corresponding fold change. (D) IL-6 mRNA expression with corresponding fold change. GAPDH was used as an internal control. Data are presented as mean ± SD (n = 3). ### p < 0.001, compared with control and * p < 0.05, ** p < 0.01, and *** p < 0.001 compare with LPS group by one-way ANOVA.