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. 2021 May 12;26(10):2870. doi: 10.3390/molecules26102870

Figure 4.

Figure 4

Effect of LOS-ME on NF-κB p65 phosphorylation in LPS-activated BV2 cells. BV2 microglia cells were treated with LOS-ME (200 μg/mL) and LPS (200 ng/mL) for 30 min and confirmed by Western blot. (A) protein expression of p65 and p-p65 with corresponding fold change. (B) BV2 microglia cells were treated with LOS-ME (200 µg/mL) and LPS (200 ng/mL) for 30 min and confirmed by ICC assay. Translocation of p65 protein was determined using an anti-p65 antibody and Alexa Fluor 568-labeled goat anti-mice antibody on immunofluorescence. Data are presented as mean ± SD (n = 3). ## p < 0.01 compared with control group, ** p < 0.01 compared with LPS group by one-way ANOVA.