Figure 5.

Effect of LOS-ME on ERK, JNK, and p38 phosphorylation in LPS-activated BV2 cells. BV2 microglia were treated with LOS-ME (25, 100, 200 μg/mL) and LPS (200 ng/mL) for 30 min and confirmed by attaching each phospho-form antibody. (A) Protein expression of ERK with corresponding fold change. (B) Protein expression of JNK and p38. The total form of each ERK, JNK, and p38 was used as an internal control. Data are presented as mean ± SD (n = 3). ^### p < 0.001 compared with control. * p < 0.05 and *** p < 0.001 compared with LPS group by one-way ANOVA.