Table 2.
Primers used for real-time PCR analyses. IL, interleukin; Tnfα, tumor necrosis factors-α; Ppparg1α, peroxisome proliferator-activated receptor coactivator-1 α; Nrf1, nuclear respiratory factor 1; Gapdh, glyceraldehyde 3-phosphate dehydrogenase.
| Genes | Forward Primer 5′ → 3′ | Reverse Primer 5′ → 3′ |
|---|---|---|
| Il1β | ATCTCACAGCAGCATCTCGA | TAGCAGGTCGTCATCATCCC |
| Il6 | CCACTGCCTTCCCTACTTCA | TTCTGACAGTGCATCATCGC |
| Il10 | AGAGAACCATGGCCCAGAAA | TGAGTGTCACGTAGGCTTCT |
| Tnfα | TCATCCGTTCTCTACCCAGC | TACTTCAGCGTCTCGTGTGT |
| Ppargc1α | AATGCAGCGGTCTTAGCACT | GTGTGAGGAGGGTCATCGTT |
| Nrf1 | TTCCTCAGCCTCCGTCTTCT | ACACACCTTGCACTCACACC |
| Gapdh | CATGCCATCACTGCCACTCA | GCGGCATGTCAGATCCACAA |