Table 3.

Diagnostic performance of bovine intelectin 2 immunohistochemical quantification for diagnosis of cattle with different types of PTB-associated histological lesions in their gut tissues.

ROC Analysis	AUC	p Value	CUT OFF	SE (%)	SP (%)	DV
Focal vs. Control	0.800	0.134	>167	100.00	80.00	0.90
Multifocal vs. Control	0.840	0.039	>167	100.00	80.00	0.90
Diffuse vs. Control	0.800	0.073	>22	100.00	60.00	0.80
Patent vs. Control	0.820	0.046	>167	90.00	80.00	0.85
Lesions vs. Control	0.813	0.069	>167	93.33	80.00	0.86

ROC, receiver operating characteristic, ROC analysis was used to determine the diagnostic performance of the method; AUC, area under the curve; p-value, it is the p-value of the AUC area, indicates whether the discrimination between animals with focal, multifocal, diffuse or any type of lesions and controls is significant; The cut-off point is expressed as the total number of ITLN2 positive cells per cow; SE, sensitivity; SP, specificity; DV, diagnostic value (semi-sum of the sensitivity and specificity); VS., versus. C, control type consisting of five animals with no lesions detected; The number of animals analyzed in the focal, multifocal and diffuse types were also five (n = 5 per group). The patent type includes the five animals with multifocal lesions and the five animals with diffuse lesions (n = 10). The discriminatory power of each biomarker to discern between the different histopathological types and the control type was classified as follows: AUC values ≥ 0.9 were considered to have excellent discriminatory power; 0.8 ≤ AUC < 0.9 good discriminatory power; 0.7 ≤ AUC < 0.8 fair discriminatory power; and AUC < 0.7, poor discriminatory [63,64].