Figure 4.

Population of CD4+ T cell subsets in mesenteric lymph node (MLN) of weaned piglets fed alternative form of zinc oxide. Forward scatter and side scatter were used to identify lymphocytes among live cells. Figures represent dot plots and graphs of CD4+ T cell (A,B) and its subsets (C,D) in piglets fed different forms of zinc oxide. CD4+ T cell subsets were identified by using the transcription factors T-bet, GATA3, RORγt, and FoxP3; T-bet, RORγt, and FoxP3 were gated simultaneously. Data are represented as mean ± SD. Values were statistically analyzed by unpaired t-test. * p < 0.05.