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. 2021 May 11;11(5):1356. doi: 10.3390/ani11051356

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Barrier function in the colon tissues of weaned piglets fed different forms of zinc oxide. The gene expression of barrier functioning (A) and antimicrobial peptides (B) in colon tissues are shown. The mRNA expressions of the tight-junction related genes, ZO-1, OCLN, and CLDN4, were analyzed. The mRNA expressions of the antimicrobial peptide genes pBD1 and REGIIIγ were analyzed. Gene expression levels of each group were calculated based on that of the negative control group (CON). All fold change values were normalized by GAPDH. Data are represented as mean ± SD. Values were statistically analyzed by one-way ANOVA with Tukey’s multiple comparison test.