Figure 1.

Metaphases of B+ (a–c) and B− (d–f) Astyanax scabripinnis sequentially treated with anti-5-mC (a,d), FISH with an As51 probe (b,e), and localization of heterochromatin regions using the C-banding technique (c,f). The B chromosome is indicated by green arrow It is possible to observe an evident marking in the telomeric region of another chromosomal pair, both in the B+ and B− genomes, strongly marked by As51 probes (b,e). One of the pairs of complement A is probably related to the origin of the B chromosome of A. scabripinnis. Bar = 10 µm.