Table 3.
Determination of seven biogenic amines in wheat germ extracts by reversed-phase high-performance liquid chromatography.
| Concentration (mg/kg) | |||||||
|---|---|---|---|---|---|---|---|
| Extract | Tryptamine | Putrescine | Cadaverine | Histamine | Tyramine | Spermidine | Spermine |
| Acetone | 47.26 ± 1.39 b | 115.59 ± 0.80 e | 65.34 ± 1.75 a | 49.45 ± 0.22 b | 36.96 ± 1.21 b | 60.46 ± 0.64 b | 45.69 ± 0.56 b |
| Ethanol | 29.01 ± 0.14 d | 126.19 ± 0.97 d | 50.34 ± 1.27 c | 49.06 ± 0.19 b | 40.02 ± 0.24 a | 23.81 ± 0.45 c | 40.61 ± 0.21 c |
| Hexane | 23.70 ± 0.56 e | 194.16 ± 2.01 a | 54.82 ± 4.01 b | 50.16 ± 0.57 a | 35.67 ± 0.67 c | 17.71 ± 0.79 e | 38.56 ± 0.12 c |
| HWE | 39.14 ± 0.16 c | 152.75 ± 0.32 b | ND | ND | 16.45 ± 0.03 e | 234.91 ± 1.35 a | 62.87 ± 1.40 a |
| SWE | 9.22 ± 0.00 f | 137.27 ± 0.57 c | 34.05 ± 0.05 d | 24.76 ± 0.04 c | 17.57 ± 0.04 d | 19.54 ± 0.04 d | 13.41 ± 0.07 d |
| UWE | 229.35 ± 0.22 a | 99.25 ± 0.93 f | 16.47 ± 1.29 e | ND | ND | 11.59 ± 0.11 f | 7.73 ± 0.50 e |
The values are expressed as mean ± standard deviation (n = 3). Different letters in each column indicate statistically significant differences at p < 0.05 (Duncan’s multiple range test). ND, not detected; HWE, hot water extract; SWE, subcritical water extract; UWE, ultrasonic water extract.