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Effects of F16-betulin conjugate on mitochondrial membrane potential. Additions: 5 µM of F16-betulin (shown by an arrow) in the presence of glutamate plus malate (red line) and succinate plus rotenone (blue line). 1 μM carbonyl cyanide-p-trifluoromethoxyphenylhydrazone (FCCP) added at the end of the recordings to completely depolarize the inner mitochondrial membrane. A similar pattern was observed in three independent experiments.
