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. 2021 May 11;22(10):5088. doi: 10.3390/ijms22105088

Figure 8.

Figure 8

qRT-PCR validation of 15 miRNAs: (a–o) Represented different miRNAs expression levels with deep sequencing and qRT-PCR. The TPM value was taken as the relative expression level of deep sequencing miRNAs, which was shown as the red line, corresponding to the Y-axis on the right side. The qRT-PCR validation used the black bar chart, corresponding to the Y-axis on the left side, and U6 was used for each sample as an endogenous control. Three independent biologicals and three technical replicates were employed; the error bars show the SD (standard deviations). The significant difference was compared using the Student’s t-test. * p ≤0.05; ** p ≤ 0.01.