Table 2.
Characteristics of fibroblastic reticular cells isolation and their immunophenotype.
| Ref. | Lymph Node Region | Digestion Type | Digestion Solution | FRC Culture Medium + Supplement | FRC Immunophenotypic Characterization | Technique for Cell Separation | Purity (%) |
|---|---|---|---|---|---|---|---|
| Aparicio-Domingo et al. [18] | Axillary; brachial; inguinal | Enzymatic | Collagenase IV; DNase I; CaCl2 | DMEM (2% FCS) | CD45; CD31; PDPN | Cell sorting | >94 |
| Dertschnig et al. [19] | Peripheral; mesenteric | Enzymatic | DNase; Liberase | NC | CD45; CD31; PDPN | Cell sorting | NR |
| Eom et al. [20] | Axillary; inguinal; cervical; mesenteric; mediastinum | Enzymatic | DNase I; Liberase DH | RPMI-1640 | CD45, CD31, PDPN | NR | NR |
| Gonzalez et al. [21] | Skin-draining (brachial; axillary; inguinal); Pancreatic | Enzymatic | Collagenase P; DNase I; Dispase II | NR | CD45; CD31; PDPN | Cell sorting | NR |
| Knop et al. [22] | Peripheral; mesenteric | Enzymatic | Collagenase P; Dispase II; DNase I; Latrunculin B | RPMI-1640 | CD45; CD31; PDPN | Cell sorting | >73.3 |
| Perez-Shibayama et al. [23] | Inguinal | Enzymatic | Collagenase F; DNase I | RPMI | NR | NR | NR |
| Brown et al. [24] | NR | Enzymatic | Collagenase P; DNase I; Dispase | α-MEM | CD45; CD31; PDPN | Cell sorting | >95 |
| Kasinath et al. [25] | Kidney | NA | NA | NA | NA | NA | NA |
| Kelch et al. [26] | Popliteal; mesenteric; Inguinal | NA | NA | NA | NA | NA | NA |
| Majumder et al. [27] | Mesenteric; inguinal | Enzymatic | DNase I; Liberase; Dispase | RPMI | CD45; CD31; PDPN; | Microbeads isolation | >98 |
| Masters et al. [28] | Mesenteric; popliteal |
Enzymatic | Liberase TL; Benzonuclease | RPMI-1640 | CD45; CD31; PDPN | Microbeads isolation | >90 |
| Schaeuble et al. [29] | Peripheral (axillary, brachial, inguinal) | Enzymatic | Collagenase IV; DNase I | DMEM (2% FCS) | CD45; CD31; PDPN | Microbeads isolation | ≥90 |
| Dubrot et al. [30] | Skin-draining | Enzymatic | Collagenase D; DNase I | HBSS | CD45; CD31; PDPN | Cell sorting | NR |
| Knoblich et al. [31] | NR | Enzymatic | Collagenase P; DNase I; Dispase | α-MEM (10% FBS) | CD45; PDPN | NR | 99 |
| Maaraouf et al. [32] | Kidney | Enzymatic | Collagenase P; DNase I; Dispase II | DMEM (10% FBS) | CD45; CD31; PDPN | NR | NR |
| Chung et al. [33] | Peripheral (cervical, axial, brachial, inguinal) | Enzymatic | Collagenase IV; DNase I | DMEM (2% FBS) | CD45; CD31; PDPN | Cell sorting | NR |
| Gao et al. [34] | Inguinal | Enzymatic | Collagenase IV; DNase I | RPMI-1640 (2% FBS) | CD45; CD31; PDPN | NA | NA |
| Pazstoi et al. [35] | Mesenteric | Enzymatic | Collagenase P; Dispase; DNase I | RPMI-1640 | CD45; CD31; PDPN | Cell sorting | 91–97 |
| Valencia et al. [36] | Mesenteric | Mechanical disruption | NR | RPMI-1640 | CD45, CD31, PDPN | NR | NR |
| Yu, M. et al. [37] | Axillary; brachial; inguinal | Enzymatic | Collagenase P; Dispase; DNase I | DMEM (10% FBS) | CD45; CD31; PDPN | Cell sorting | >95 |
| Gil-Cruz et al. [38] | Mesenteric | Enzymatic | Collagenase D; DNase I | RPMI-1640 (2% FCS) | CD45; CD31; PDPN | Cell sorting | NR |
| Novkovic et al. [39] | Inguinal | Enzymatic | Collagenase P; DNase I | RPMI (2% FCS) | PDPN | NA | NA |
| Royer et al. [40] | Mandibular | Mechanical disruption | NR | RPMI-1640 (10% FBS) | NR | NR | NR |
| Takeda et al. [41] | Mesenteric; peripheral; brachial | Enzymatic | Collagenase P; Dispase; DNase I | RPMI-1640 | CD45; CD31; PDPN | Cell sorting | NR |
Abbreviations—Ref.: reference; NR: not reported; NA: not applicable; FBS: fetal bovine serum; FCS: fetal calf serum; PDPN or gp38: podoplanin; NC: not cultivated.