Table 3.
Characteristics of the main type of immune cells used for analysis with fibroblastic reticular cells.
| Ref. | Source of Cells | Cell Type | Separation Technique | Immune Cell Preservation Solution and Supplementation | Immune Cell Immunophenotypic Characterization |
|---|---|---|---|---|---|
| Aparicio-Domingo et al. [18] | LN | CD8+ T cells | Non selection performed | DMEM (2% FCS) | CD45, CD8α, CD4, TRCαβ |
| Dertschnig et al. [19] | LN | T cells | CD3 negative selection followed by CD4 and CD8α positive selection (MicroBeads—Myltenyi) |
NR | CD45, CD45.1, CD3, CD4, CD8α, CD62L, CD44, CD69, CD127, Vα2, Vβ5 |
| Eom et al. [20] | LN | NA | NA | NA | CD45, CD3, CD8 |
| Gonzalez et al. [21] | Spleen | CD8+ T cells | CD8 isolation by negative selection (Microbeads—MojoSort) | NR | CD45, CD8, CD44, CD25 |
| Knop et al. [22] | LN; spleen | T cells and NK | CD8α positive selection (MicroBeads—Myltenyi) | RPMI | CD45, CD3, CD4, CD5, CD8α, CD62L, Bcl-2, CD127, Nk1.1, RORγt |
| Perez-Shibayama et al. [23] | LN; spleen | T cell subsets and exhaustion | No selection performed | RPMI | CD45.1, CD45.2, CD45R, CD8α, CD8β, CD3e, CD44 CD62L, PD-1, PDL1 |
| Brown et al. [24] | LN | CD8+ T cells | CD8α positive selection (MicroBeads—Myltenyi) | RPMI; α-MEM | CD45.1, CD45.2, CD3, CD4, CD8, CD275, CD28, CD44 |
| Kasinath et al. [25] | LN; spleen | CD4+ T cells | No selection performed | NR | CD45, CD3, CD4, CD44, CD62L, IL-17A |
| Kelch et al. [26] | LN | NA | NA | NA | NA |
| Majumder et al. [27] | LN | T and B cells | NR | NR | CD45, CD45.2, CD4, B220, IL-17A, IL-17R |
| Masters et al. [28] | LN; peripheral blood | CD8+ T | CD8 isolation by negative selection (Microbeads—MojoSort) | NR | CD45, CD45.1, CD45.2, CD69, CD8α |
| Schaeuble et al. [29] | LN; spleen | T cells | No selection performed | RPMI | CD45, CD3, CD4, CD8α, CD44, CD62L, CD279, FoxP3, CD25 |
| Dubrot et al. [30] | LN; spleen | T cells, B cells, Treg, and DC | Pan T isolation by negative selection (MicroBeads—Myltenyi) |
NR | CD45, CD44, CD3, CD4, CD8α, FOXp3, Ly5.1, CD11b, CD19, CD25, CD62L, PDCA-1, PD-1, IL-17, IFNγR |
| Knoblich et al. [31] | LN; tonsils | T cells | Pan T isolation by negative selection (MicroBeads—Myltenyi) |
NR | CD45, CD3, CD4, CD8, CD62L, CD27, CD45RO, CD25 |
| Maaraouf et al. [32] | Spleen | T cells | Pan T isolation by negative selection (MicroBeads—Myltenyi) |
NR | CD45, CD4 |
| Chung et al. [33] | Spleen; peripheral blood | T cells, B cells, FDCs, Treg, and DCs | T cell Thy.1 selection (Microbeads—StemCells Technologies) |
NA | CD45, CD3, CD4, CD8, FOXp3, CD157, CD19, B220, CD44, CD62L, CD11c, CD11b, CD169, CD21/35, F4/80, TCRβ |
| Gao et al. [34] | LN | T cells | NR | NR | CD45, CD4, CD8 |
| Pazstoi et al. [35] | LN | T cells | CD4 positive selection (Microbeads—Myltenyi) |
EX VIVO | CD45, CD45.2, CD4, CD2, CD9, CD24, CD25, CD63 |
| Valencia et al. [36] | LN | CD4+ T cells | CD4 naïve T cell negative selection (Microbeads—Myltenyi) |
RPMI (10% FCS) | CD45, CD44, CD4 |
| Yu, M. et al. [37] | LN | T cells | Pan T cell negative selection (Microbeads—StemCells Technologies) |
RPMI (10% FBS) | CD45, CD45.1, CD45.2, CD3, CD4, CD8α, CD25, CD69, CD44 |
| Gil-Cruz et al. [38] | PP; LN | T cells, B cells, NK cells, Treg, and ILCs | NR | RPMI (10% FCS) | CD45, CD3e, CD4, CD8α, EOMES, FoxP3, B220, CD19, CD127, CD62L, CD44, CD69, F4/80, IL-17A, IL-7Rα, GATA3, RORγt, IL-15RαIL-15Rβ, NKp46, NK1.1 |
| Novkovic et al. [39] | LN; Spleen | DCs and T cells | NR | RPMI (2% FCS) | CD45, CD3, CD8, CD4, CD11c, MHCII |
| Royer et al. [40] | SLOs | CD8+ T cells | CD8 positive selection (Microbeads—Myltenyi) | RPMI (10% FBS) | CD45, CD3, CD4, CD8 |
| Takeda et al. [41] | LN; Spleen | T cells, B cells | CD4 naïve T cell negative selection (Microbeads—Myltenyi) |
RPMI | CD4, CD8, B220, CD44 |
Abbreviations:—Ref.: reference; NR: not reported; NA: not applicable; FCS: fetal calf serum; PP: Peyer’s Patches; LN: lymph nodes; SLOs: secondary lymphoid organs; DCs: dendritic cells; NK: natural killer cells.