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N-CLCA1 increases mucus production in vivo. (A) Semiquantitative RT-PCR analysis of the expression of MUC5AC, TMEM16A, CFTR, SLC26A9 and KCNN4 in isolated tracheal epithelial cells from control mice and mice treated for 24 h with N-CLCA1. Mean ± SEM (number of animals/number of reactions). #—significant difference when compared to control or mock (p < 0.05; unpaired t-test). (B) Alcian blue staining indicating enhanced mucus secretion in airways exposed to N-CLCA1. Bar = 100 µm.
