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. 2021 May 7;10(5):742. doi: 10.3390/antiox10050742

Table 4.

In vitro protein and starch digestibility and nutritional indexes of pasta containing fermented and native brewers’ spent grain: BSG-p, pasta containing native brewers’ spent grain (15% wt/wt in replacement of semolina); fBSG-p, pasta containing fermented brewers’ spent grain (15% wt/wt in replacement of semolina); W-p, pasta made with durum wheat semolina.

BSG-p fBSG-p W-p
Nutritional indexes
In Vitro Protein Digestibility (IVPD) % 78.65 ± 1.03 b 88.52 ± 0.05 a 76.22 ± 1.01 b
Chemical scores (%)
Histidine 40.6 ± 1.1 b 43.4 ± 0.7 a 38.8 ± 1.0 b
Isoleucine 55.4 ± 1.4 b 58.7 ± 1.4 a 39.9 ± 0.5 c
Leucine 77.1 ± 2.9 b 83.1 ± 3.4 a 61.3 ± 1.5 c
Lysine 23.8 ± 0.7 b 28.6 ± 0.3 a 21.4 ± 0.5 c
Cysteine 55.7 ± 0.2 c 63.6 ± 0.1 b 73.7 ± 0.6 a
Methionine 48.01 ±1.5 a 51.5 ± 1.0 a 51.0 ± 1.0 a
Phenylalanine + Tyrosine 82.5 ± 2.8 b 86.1 ± 3.1 a 78.8 ± 1.8 c
Threonine 27.2 ± 1.1 b 31.5 ± 0.9 a 33.3 ± 1.1 a
Valine 41.3 ± 2.6 b 44.8 ± 0.2 a 45.8 ± 1.2 a
Tryptophan 42.4 ± 3.1 b 46.2 ± 0.7 a 42.1 ± 0.2 b
Sequence of limiting EAA
Lysine Lysine Lysine
Threonine Threonine Threonine
Histidine Histidine Histidine
Essential Amino Acid Index (EAAI) 45.7 ± 1.1 b 50.3 ± 0.8 a 45.2 ± 0.7 b
Biological Value (BV) 38.1 ± 0.5 b 43.1 ± 0.2 a 37.5 ± 0.1 b
Protein Efficiency Ratio (PER) 32.7 ± 0.4 a 32.7 ± 0.7 a 32.7 ± 0.5 a
Nutritional Index (NI) 1.3 ± 0.1 b 2.5 ± 0.1 a 1.3± 0.1 b
Hydrolysis Index (HI) 63.46 ± 1.99 b 58.86 ± 1.25 c 68.65 ± 1.47 a
Predicted Glycemic Index (pGI) 74.55 ± 1.10 b 72.02 ± 0.68 c 77.35 ± 0.81 a

The data are the means of three independent experiments ± standard deviations (n = 3). a–c Values in the same row with different superscript letters differ significantly (p < 0.05).