Table 1.
Strains and plasmids used in the current study.
| Strains or Plasmids | Relevant Characteristic | References or Source |
|---|---|---|
| Strains | ||
| S17, CICC1929 | Wild-type top-fermenting industrial brewer’s strain | This study |
| E. coli DH5α | Host of plasmid | Stratagene |
| Transformants | ||
| SST1 | S-17GAT1(n-1)/ΔGAT1::loxP-KanMX-loxP | This study |
| SST1K | S-17GAT1(n-1)/ΔGAT1::loxP::loxP | This study |
| SDT1 | S-17GAT1(n-2)/ΔGAT1::loxP-KanMX-loxP | This study |
| SDT1K | S-17GAT1(n-2)/ΔGAT1::loxP::loxP | This study |
| SSP1 | S-17GAP1(n-1)/ΔGAP1::loxP-KanMX-loxP | This study |
| SSP1K | S-17GAP1(n-1)/ΔGAP1::loxP::loxP | This study |
| SDP1 | S-17GAP1(n-2)/ΔGAP1::loxP-KanMX-loxP | This study |
| SDP1K | S-17GAP1(n-2)/ΔGAP1::loxP::loxP | This study |
| SSA9 | S-17ARO9(n-1)/ΔARO9::loxP-KanMX-loxP | This study |
| SSA9K | S-17ARO9(n-1)/ΔARO9::loxP::loxP | This study |
| SDA9 | S-17ARO9(n-2)/ΔARO9::loxP-KanMX-loxP | This study |
| SDA9K | S-17ARO9(n-2)/ΔARO9::loxP::loxP | This study |
| SSA10 | S-17ARO10(n-1)/ΔARO10:: loxP-KanMX-loxP | This study |
| SSA10K | S-17ARO10(n-1)/ΔARO10::loxP::loxP | This study |
| SDA10 | S-17ARO10(n-2)/ΔARO10::loxP-KanMX-loxP | This study |
| SDA10K | S-17ARO10(n-2)/ΔARO10::loxP::loxP | This study |
| Plasmids | ||
| pUG6 | Kan r, containing loxP-KanMX-loxP disruption cassette | [18] |
| pSH-Zeocin | Zeo r, Cre recombinant enzyme expression vector | [19] |