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. 2021 May 11;13(10):2293. doi: 10.3390/cancers13102293

Figure 1.

Figure 1

YUMMER (ER) and YUMM (Y) cell lines have different transcriptomes in vitro and establish synchronous melanoma tumors in vivo. (a) PCA plot separating overall transcriptomic differences between ER and Y cell lines in vitro. Notice how the two cell lines generate distinct groupings along the PC1 axis. (b) Volcano plot highlighting differentially expressed genes in ER (red) vs. Y (blue) cell lines. Colored genes are statistically significant with adjusted p-value < 0.05 and log 2-fold change > |1|. (c) Top-most upregulated pathways in ER and Y cell lines as identified by IPA analysis. Red bars indicate pathways upregulated in ER and blue bars indicate pathways upregulated in Y cells. (d) Synchronous murine melanoma model schematic. The analyzed tumor in the synchronous model is underlined and bolded in subsequent figures. A table of tumor combination can be found in Supplementary Table S1. (e) Growth curves of individual ER (ER + Y) and Y (ER + Y) tumors in synchronous melanoma mice. (f) Percent of ER tumors rejected and growth curve (g) of synchronous ER tumors from ER + Y, ER + ER, and ER + B16 mice. Data (mean ± SEM) in (e–g) are pooled, from 3–5 mice/group/experiment, and representative of at least 2 independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, n.s. not significant.