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. Author manuscript; available in PMC: 2021 May 26.
Published in final edited form as: Acta Biomater. 2017 Aug 17;62:222–233. doi: 10.1016/j.actbio.2017.08.022

Figure 2. Material characterization of Alg-G-H.

Figure 2.

A) Rheological properties of unmodified alginate and Alg-G-H. G’ (storage modulus) and G” (loss modulus) are shown for 3 concentrations of alginate and Alg-G-H hydrogels. B) Images of MSCs seeded on alginate or Alg-G-H hydrogels, verified successful incorporation of the gelatin component in Alg-G-H material. Because an increasing relative ratio of gelatin-to-alginate is used in the chemical synthesis, the resulting hydrogel material is more successful at supporting cell adhesion. Scale bar=100 μm. C-E) Morphological assessment of cells seeded on alginate and Alg-G-H hydrogels. Cultures were assessed for C) percentage of adherent and spreading cells, D) eccentricity, and E) filopodia length. In each condition, a higher relative value indicates increased ability to attach and interact with the hydrogel. F) Proliferation of MSCs on alginate and different gelatin concentrations of Alg-G-H hydrogels up to 7 days. Data were shown based on 3 independent experiments with 3 replicates as mean ± SEM, One-way analysis of variance (ANOVA) and Student’s t-test were used for statistical calculation: * p < 0.01 in comparisons between Alg-G-H 33% gelatin versus all other substrates.