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. Author manuscript; available in PMC: 2021 May 26.
Published in final edited form as: Acta Biomater. 2017 Aug 17;62:222–233. doi: 10.1016/j.actbio.2017.08.022

Figure 6. Myogenic differentiation of hSMPCs on different substrates within 2 weeks.

Figure 6.

Myotube formation and multinuclear fusion on Matrigel®, HA-HP-ECM, and Alg-G-H-ECM coating conditions, compared with the gel with no ECM or uncoated condition as control, detected by immunofluorescent staining after 2-week differentiation with A) MyoD, B) Myf5 in the initial stage of myogenesis, C) Myogenin in the intermediate stage of myogenesis, D) Desmin, and E) Myosin in the maturation stage of myogenesis. All the differentiated cells positively stained with myogenesis markers performed green fluorescent, nuclei stained with propidium iodide (PI, red). Representative images are shown from 3 independent experiments with at least 3 replicates. Scale bar=100 μm.