Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 May 7;57(5):456. doi: 10.3390/medicina57050456

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

(a). A2780 monolayer cells stained for acetylated histones H2A and H2B. The light microscopic images of A2780 cells after SAHA (7.5 µM) treatment in the upper panels. The results of immunocytochemical detection of Ac-H2A and Ac-H2B are shown in the lower panels (green fluorescence) and visualized using Alexa Fluor^® 488. The monolayer cells were observed under Leica (DMI3000 B) microscope using 10× magnification. (b). A2780 monolayer cells stained for acetylated histones, including H3 and H4. The light microscopic images of A2780 cells after SAHA (7.5 µM) treatment in the upper panels. The results of immunocytochemical detection of Ac-H3 and Ac-H4 are shown in the lower panels (green fluorescence) and visualized using Alexa Fluor^® 488. (c). The A2780 monolayer cells of were stained for p21. The light microscopic images of A2780 cells after SAHA (7.5 µM) treatment in the upper panels. The results of immunocytochemical detection of p21^WAF1/CIP1 levels are shown in the lower panels (green fluorescence) and visualized using Alexa Fluor^® 488.

(a). A2780 monolayer cells stained for acetylated histones H2A and H2B. The light microscopic images of A2780 cells after SAHA (7.5 µM) treatment in the upper panels. The results of immunocytochemical detection of Ac-H2A and Ac-H2B are shown in the lower panels (green fluorescence) and visualized using Alexa Fluor^® 488. The monolayer cells were observed under Leica (DMI3000 B) microscope using 10× magnification. (b). A2780 monolayer cells stained for acetylated histones, including H3 and H4. The light microscopic images of A2780 cells after SAHA (7.5 µM) treatment in the upper panels. The results of immunocytochemical detection of Ac-H3 and Ac-H4 are shown in the lower panels (green fluorescence) and visualized using Alexa Fluor^® 488. (c). The A2780 monolayer cells of were stained for p21. The light microscopic images of A2780 cells after SAHA (7.5 µM) treatment in the upper panels. The results of immunocytochemical detection of p21^WAF1/CIP1 levels are shown in the lower panels (green fluorescence) and visualized using Alexa Fluor^® 488.