Table 1.
Characterized key enzymes in the taxol pathway.
| Enzyme Abbreviation | Size (kDa) | Probable Localization | GenBank Number |
|---|---|---|---|
| Geranylgeranyl diphosphate synthase GGPPS | 42 | Plastids | AF081514 |
| Taxadiene synthase TS | 98 | Plastids | AY364469 |
| Taxadiene-5α-hydroxylase T5αOH | 56 | Endoplasmic reticulum | AY289209 |
| Taxane-10β-hydroxylase T10βOH | 56 | Endoplasmic reticulum | AF318211 |
| Taxane-10β-hydroxylase a T10βOH | 55 | Endoplasmic reticulum | AY563635 |
| Taxadiene-13α-hydroxylase T13αOH | 54 | Endoplasmic reticulum | AY056019 |
| Taxane-2α-hydroxylase T2αOH | 55 | Endoplasmic reticulum | AY518383 |
| Taxane-9α-hydroxylase T9αOH | 55 | Endoplasmic reticulum | KF773141 |
| Taxane-7β-hydroxylase T7βOH | 56 | Endoplasmic reticulum | AY307951 |
| Taxadiene-5α-ol-O-acetyl transferase TAT | 49 | Cytosol | AF190130 |
| Taxane-2α-O-benzoyl transferase TBT | 50 | Cytosol | AF297618 |
| 10-deacetylbaccatin III-10-O acetyl transferase DBAT | 49 | Cytosol | AF193765 |
| Baccatin III: 3-amino, 13-phenylpropanoyltransferase BAPT | 50 | Cytosol | AY082804 |
| N-benzoyl transferase DBTNBT | 49 | Cytosol | AF466397 |
| Phenylalanine aminomutase PAM | 76 | Cytosol | AY582743 |
| β-phenylalanyl-CoA ligase b PCL | 59 | Cytosol | KM593667 |
a A second taxane-10β-hydroxylase has also been found; b The PCL listed here is a putative candidate isolated from T. baccata cultures.