Figure 5.

Investigation of RBC shapes in microfluidic flow. (A) Panel A shows the flow shapes of healthy RBCs (normocytes) on the left and a variety of acanthocytes on the right. (B) Panel B compares representative phase diagrams of a healthy control donor on the left and an (untreated) ChAc patient on the right, with the fitted exponential changes in the cell numbers. The transition point, where the number of croissants and slippers is identical, is marked with brown circles. (C) Panel C compares the transition points of a cohort of healthy controls and untreated ChAc pa-tients, indicating a higher rigidity of the normocytes in ChAc patients. * refers to a significance level of p < 0.05. (D) Panel D depicts the variance of the deformation parameter, d, of the acan-thocytes of different donors in flow.