Figure 4.

Schematic protocol of rat arterial blood pressure (ABP) measurements. (A) Illustration of an anesthetized rat with a catheter inserted in right femoral artery for ABP measurements through a blood pressure transducer, and with a catheter inserted in left femoral vein for i.v. injections. Rat drawing modified from Watts et al. [47]. (B) Schematic time-course of the protocol. Each rat was challenged every 5 min with AngII, ACh and vehicle solution (VEH) before fixed concentrations of NIC, ACE, CLO or DMSO 2% (CTRL). (C) Analysis method of raw trace to collect the difference between mean arterial blood pressure ($\Delta$ABP). See Materials and Methods Section 4.7 for further explanation.