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. 2021 May 11;22(10):5083. doi: 10.3390/ijms22105083

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Immunohistochemical analysis of rod and cone photoreceptors in transgenic (Tg:XOPS) wild-type and exoc5 mutant zebrafish. Top panel: wild-type:Transgenic-XOPS (WT;Tg-XOPS-GFP) zebrafish retinas immunostained with R/G cone opsin (red). Bottom panel: exoc5^−/− mutant:Transgenic-XOPS (exoc5^−/−;Tg-XOPS-GFP) retinas immunostained with R/G cone opsin (red). The transgenic line expresses soluble endogenous GFP in rods (green). Significantly shorter rod and cone OS were observed in exoc5^−/− mutant zebrafish, compared to WT zebrafish, at similar ages. (C) Image J was used to quantify fluorescence for R/G cone opsin and GFP+ rods. ** p < 0.005 (WT vs. exoc5^−/− mutants).